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8. Press EVAC on SC AIRLOCK VALVE DISPLAY - this will pump the specimen
exchange chamber.
9. Observe SEC vacuum display as status lights show vacuum goes from AIR to LOW to
HIGH.
10. When SEC vacuum is FilCH (green light is on), open airlock door, by turning airlock level
anticlockwise about one half turn. DO NOT FORCE THIS LEVER, it requires minimal
effort to open.
11. When airlock door is open, fully insert specimen exchange rod into specimen chamber.
Watch the specimen being inserted and ensure that it sits properly inside the specimen chamber.
When rod is fully inserted, unscrew rod from sample by turning rod anticlockwise.
12. When rod is unscrewed, withdraw the rod into the specimen exchange chamber.
13. Close airlock door by turning airlock level clockwise about one half turn.
C TURNING THE BEAM ON – GETTING AN IMAGE
1. Select the required accelerating voltage Press PF1 Vacc
2. Using numeric keyboard key in desired accelerating voltage e.g. 5kv.
3. Press ENTER
4. Microscope will automatically go to 5kV.
5. Press PF16 EXIT to clear screen
6. Press the ON bunon on the HV display (middle button). The red left hand button will flash
while the high tension comes on. When the beam current has been reached the light will stop
flashing.
7. Set magnification to IQOOX. You can do this by manually turning the
MAGNIFICATION knob or pressing the PRESET switch on the magnification display.
8. Locate area of interest using X and V stage shifts (you may need to go to low magnification
see section I).
9. Turn the COARSE FOCUS knob until a nominally focused image is obtained.
10. Press ABC button on the image adjust section to automatically correct brightness and
contrast.
#
11. Press SMALL AREA DISPLAY button on scan speed section.
12. Increase magnification to -10,000 times by manually turning the MAGNIFICATION
knob.
13. Focus using FINE FOCUS knob
14. Refine sharpness of image with STIGMA/ALIGNMENT X AND Y knobs
15. Return to TV or SLOW SCAN MODES on scan speed section.
16. If you wish to move the sample to a different area, manipulate the specimen with the X
AND Y shifts on the specimen chamber. You can also adjust the height, Z tilt, T and rotation,
R of the sample. If you adjust the tilt or height of the sample, you will need to consider the
effects of that movement with respect to any detectors inserted into the chamber. Always return
X , Y, Z , R and T controls to their preset levels before removing a sample.
11. When you have moved to a new area you may need to repeat steps 9-16.
# The ABC control provides 'ideal brightness and contrast levels for photography. But may not always
provide the levels of brightness and contrast you desire. An alternative method is to use the
BRIGHTNESS and CONTRAST up/down arrowed keys on the image adjust section.