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19
Protocol
9. Stop the reaction by the
addition of 5 µl of 0.2 M
EDTA. For use in a
hybridization, denature the
labelled DNA by heating to
95-100°C for 5 minutes, then
chill on ice continued.
Notes
9. Continued
described in Appendix III.
Calculation of probe specific
activity is described in
Appendix II.
Extensive experimentation
with Rapid-hyb buffer
(RPN1635/6) has shown that
probe purification, even
under the conditions given
above is not required with
the isotopes
32
P and
32
P.
Purification of
32
S labelled
probes is however required
to reduce filter background.