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c) Probe length
i) One labelled dNTP
ii) Two labelled dNTP
iii) Three labelled dNTP
Figure 3. The use of [α–
32
P]dNTPs in the Megaprime DNA labelling
system (see below).
Notes to figure 3
a. The results shown are the means of a number of experiments in
which different nucleotides and combinations of nucleotides
were used. Observed results may deviate ± 10% from those
shown.
b) As the number of different labelled nucleotides is increased, at a
given level of total input label, the net synthesis of DNA is reduced.
Although the overall incorporation efficiency is reduced the
labelled product is of a higher specific activity.
Mean probe length (bases)
100
80
60
40
20
(i)
(ii)
(iii)
0 10 20 30 40 50 60 70 80 90 100
Total input label (pmols)