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6. Wash the pellet once in 90% ethanol, in the same manner. Dry the
pellet.
7. Finally redissolve the DNA pellet in TE buffer for use as a probe
and for storage.
6.4. Appendix IV. Additional equipment and
reagents
TE buffer (10 mM Tris/HCl, pH 8.0, 1 mM EDTA)
0.2 M EDTA solution
Adjustable pipettes for example Pipetman™
Sterile pipette tips
Waterbaths at 37°C and 100°C
Polypropylene microcentrifuge tubes
Microcentrifuge
Gloves
Radiation safety equipment
DE81 ion-exchange chromatography paper (Whatman)
Trichloroacetic acid (TCA) solution: 10% (w/v) TCA in water
Filter discs; glass fibre or nitrocellulose
Plastic or siliconized glass tubes, capacity ~5 ml
Filtration apparatus
2x SSC (30 mM Na
3
citrate, 300 mM NaCl, pH 7.0)
™ Pipetman is a registered trademark of Gilson
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