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3. System components
Magaprime DNA RPN1604 RPN1605 RPN1606 RPN1607
labelling
Primer solution: 150 µl 300 µl 150 µl 300 µl
Random nonamer
primers in an
aqueous solution
Labelling buffer; – – 300 µl 600 µl
dATP, dGTP and
dTTP in Tris/HCl
pH7.5,
2-mercaptoethanol
and MgCl
2
Nucleotide solutions
(a) dATP 120 µl 240 µl – –
(b) cCTP 120 µl 240 µl – –
(c) dGTP 120 µl 240 µl – –
(d) dTTP 120 µl 240 µl – –
in Tris/HCl pH8.0,
0.5 mM EDTA
Reaction buffer: 150 µl 300 µl – –
A 10x concentrated
buffer containing
Tris/HCl pH7.5,
2-mercaptoethanol
and MgCl
2
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