GE RPN1606 Label Maker User Manual


 
3. System components
Magaprime DNA RPN1604 RPN1605 RPN1606 RPN1607
labelling
Primer solution: 150 µl 300 µl 150 µl 300 µl
Random nonamer
primers in an
aqueous solution
Labelling buffer; 300 µl 600 µl
dATP, dGTP and
dTTP in Tris/HCl
pH7.5,
2-mercaptoethanol
and MgCl
2
Nucleotide solutions
(a) dATP 120 µl 240 µl
(b) cCTP 120 µl 240 µl
(c) dGTP 120 µl 240 µl
(d) dTTP 120 µl 240 µl
in Tris/HCl pH8.0,
0.5 mM EDTA
Reaction buffer: 150 µl 300 µl
A 10x concentrated
buffer containing
Tris/HCl pH7.5,
2-mercaptoethanol
and MgCl
2
6