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1. Legal 3
2. Handling 4
2.1. Safety warnings and precautions 4
2.2. Storage and stability 4
2.3. Quality control 4
3. System components 6
3.1. Megaprime DNA labelling systems 8
4. Introduction 9
5. Megaprime DNA labelling protocols 11
5.1. Standard megaprime protocol 11
5.2. New megaprime protocol 15
5.3. Use of alternative reaction conditions 20
6. Appendices 27
6.1. Appendix I. Labelling of DNA fragments in low melting
point agarose 27
6.2. Appendix II. Monitoring the reaction and calculating
the specific activity of the labelled DNA 28
6.3. Appendix III. Removal of unincorporated nucleotides 32
6.4. Appendix IV. Additional equipment and reagents 34
7. Troubleshooting guide 35
8. References 38
9. Related products 39