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c. The data was generated using the standard labelling protocols.
If dNTPs <3000 Ci/mmol are to be used, then the desired probe
specific activity must be multiplied by a conversion factor, before
determining the amount of input label.
For a single labelled dNTP:-
Total input label (pmols) = 3000 Ci/mmol x required probe
specific activity of specific activity
dNTP to be used
For more than one labelled dNTP the mean specific activity of the
labelled dNTP to be used should be inserted in the above
calculation.
Having determined the required number of pmols of input label
with reference to figure 3a, the required volume of each labelled
dNTP can be calculated. Note that the figures give the total
amount of input label required. If more than one labelled dNTP is
to be used, this figure should be divided by the number of
labelled dNTPs to be used to give the required number of pmols
of each labelled dNTP.
Volume of each labelled dNTP required in µl =
pmol of dNTP required x specific activity of dNTP (Ci/mmol) x 10
–3
radioactive concentration of dNTP (mCi/ml)
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