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3. Installation
Silicone tubing (inner diameter 8 mm) can be used over the
entire working temperature range of the unit. PVC tubing
can only be used in the temperature range of 10 to 60 °C.
1. Connect the inlet and outlet tubing to the nozzles on the
rear of the circulator (Fig. 1).
2. Connect the other ends to the electrophoresis unit. The
nozzles have an outer diameter of 9.5 mm. To ensure safe
tubing connections, secure all tubing with hose clamps.
When MultiTemp III is used as a water bath, the nozzles
should be connected to each other.
Condensation of water on cold tubing can be avoided by
covering it with insulating tubing. (See Insulation for cooling
tubing in the Ordering Information section.)
Condensation on the cooling plate of Multiphor II during
sample application can be avoided by shutting off the circula-
tion to the cooling plate with a three way valve. (See 3-way
Valve Set in the Ordering Information section.)
Note: Do not touch or remove tubing when the unit is in
operation. Stay alert to the dangers of hot water. The
surface temperature of the tubing can reach 70 °C at
high working temperatures.
Use distilled water in the temperature range of 5 to 90 °C. A
mixture of distilled water and ethylene glycol in a concentra-
tion up to 20% is suitable for lower ranges (-10 to +40 °C).
Warning! Ethylene glycol is poisonous. Handle with care and
follow recommended safety procedures.
Warning! Methanol and ethanol are not recommended becau-
se of their low flash points. Both liquids are unsafe to use.
Remove the plastic lid and fill the bath with liquid. The dis-
tance between the surface of the liquid and the stainless steel
top plate should be about 2 cm. If it is more than 4 cm, a
sensor will activate the temperature limit switch and cause
cut-off.
To drain the bath, switch off the mains power and remove
the outlet tubing from the external equipment and let the
7
3.2. Tubing
connec-
tions
3.3 Recom-
mended
bath
liquids
3.4 Filling and
draining
the bath