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Figure 1. Preparation of labelled probes using GE Healthcare’s megaprime DNA labelling systems.
Linear dsDNA
Denature in presence
of monamer primers
Add Multiprime DNA
reaction buffer
Add labelled dNTP
and ‘Klenow’ DNA
polymerase. Incubate
Denature to release
labelled probe and add
directly to hybridization
Random sequence
monamers
Unabelled dNTPs
‘Klenow’ polymerase
Labelled dNTP