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a. At the specific activity reference date of the labelled nucleotide.
b. Formulation code 1 = 370 MBq/ml, 10 mCi/ml in stabilized aqueous solution.
c. The probe specific activities were calculated using observed incorporation levels which are similar to
those found in figure 3b.
d. It is important to note that the specific activity of probes made from different amounts of labelled
dNTP cannot be calculated on a proportional basis, because net DNA synthesis occurs.
e. Brackets enclose nucleotides used in combination.
Compounds Specific activity Formulation Product Quantity of each Specific
(see note a) (see note b) code dNTP required activity of
probe
(see notes
c,d and e)
TBq/mmol Ci/mmol MBq µCi µl pmol dpm/ug
[α–
32
P]dCTP ~111 ~3000 1 PB 10205 1.85 50 5 17 1.9 x 10
9
~222 ~6000 1 PB 10475 7.4 200 20 32 5.3 x 10
9
[α–
32
P]dATP ~111 ~3000 1 PB 10204 1.85 50 5 17
3.4 x 10
9
[α–
32
P]dCTP ~111 ~3000 1 PB 10205 1.85 50 5 17
[α–
32
P]dATP ~111 ~3000 1 PB 10204 1.85 50 5 17
[α–
32
P]dCTP ~111 ~3000 1 PB 10205 1.85 50 5 17 3.7 x 10
9
[α–
32
P]dGTP ~111 ~3000 1 PB 10206 1.85 50 5 17
}
}