Filter
Top Products
5
using 17 pmol/25 ng DNA of
[α–
32
P] labelled nucleotides,
specific activity 3000 Ci/mmol
(codes PB 10204-7) and
RPN 1606/1607 are tested
using 17 pmol/25 ng DNA of
[α–
32
P]dCTP, 3000 Ci/mmol
(code PB 10205). Incorporations
greater than 55% are achieved
after 10 minutes incubation
at 37°C, as assayed by thin-
layer chromatography on PEI
cellulose in 1.25 M KH
2
PO
4
.
PH3.4.
In addition components of the
kits are checked for identity by
HPLC and the DNA solutions
for concentration by UV
spectrophotometry.