GE RPN160A Label Maker User Manual


 
Magaprime DNA RPN1604 RPN1605 RPN1606 RPN1607
labelling
Enzyme solution; 60 µl 120 µl 60 µl 120 µl
1 unit/µl DNA
polymerase 1 Klenow
fragment (cloned in
100 mM potassium
phosphate pH6.5,
10 mM 2-mercapto-
ethanol and
50% glycerol
Standard DNA 25 µl 50 µl 25 µl 50 µl
solution; 5 ng/µl
Hind III digested
lambda DNA in
10 mM Tris/HCl
pH 8.0, 1 mM
EDTA
Carrier DNA 1.25 ml 2.5 ml 1.25 ml 2.5 ml
solution; 500ng/ml
sonicated herring
sperm DNA in
10 mM Tris/HCl
pH 8.0, 1 mM
EDTA
7