Filter
Top Products
22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes
To verify sensitivity for 6120 Quad - G1978B Multimode, Positive
SIM Modes
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
flask. Use a clean graduated pipette.
2 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
acetic acid.
3 Transfer 1 mL of the first dilution to a second 50 mL volumetric flask. Use a
clean graduated pipette.
4 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
acetic acid.
This provides the final 2 pg/µL reserpine concentration required for
performance verification.
5 Transfer approximately 1 mL of the second dilution to a vial for use in the
LC autosampler.
NOTE
The G6110A instrument does not support the multimode source without the Selection
valve. Use the option kit G2735H to enable the G6110A to work with the multimode source.
Table 1 6110 and 6120 Quad Performance Verification Summary Table
G1978B
ES Positive SIM Mode
G1978B
APCI Positive SIM Mode
Sample Reserpine, 5 ng/µL Reserpine, 5 ng/µL
Concentration after dilution 2 pg/µL 2 pg/µL
Injection volume 5 µL 5 µL
Total sample amount injected 10 pg 10 pg
Sample order number G2423A G2423A
Solvent 75:25
methanol/water
with 0.1% formic or acetic acid
75:25
methanol/water
with 0.1% formic or acetic acid