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26 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes
experiments to achieve maximum instrument sensitivity
7 Review the results.
When the method is finished, a report prints showing the signal-to-noise ratio
for the five blank and five sample peaks, and a blank-subtracted average of the
sample peaks. The blank-subtracted average signal-to-noise value must be
greater than or equal to 20:1 peak-to-peak (100:1 RMS).
Sensitivity Verification for Multimode in APCI, Positive SIM Mode
1 Start the ChemStation software.
2 Change the view to MSD Tune, and start an Autotune.
After the autotune completes, you may need to wait up to 30 minutes before
you continue, to allow for the calibrant solution to be pumped out of the
Quad instrument. This minimizes any background signal resulting from the
calibrant.
3 Change the view to Method and Run Control, and load the method
6130SIMCI_MM.M (6130 model) or 6140SIMCI_MM.M (6140 model)
4 Edit the method to ensure that 75:25 methanol/water with 5mM ammonium
formate is selected as the LC solvent.
5 Place the vials into the LC autosampler.
• Position #1: empty, uncapped vial
• Position #2: vial of the solvent used for dilution (solvent blank)
• Position #3: vial with the reserpine sample (1 pg/µL)
6 Run the method.
You may need to further optimize the nebulizer pressure by running FIA
experiments to achieve maximum instrument sensitivity
The method does an FIA run with one injection of the empty vial, five
injections of the solvent blank, and five injections of the reserpine sample.
7 Review the results.
When the method is finished, a report prints showing the signal-to-noise ratio
for the five blank and five sample peaks, and a blank-subtracted average of the
sample peaks. The blank-subtracted average signal-to-noise value must be
greater than or equal to 10:1 peak-to-peak (50:1 RMS).