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30 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation
To verify sensitivity for the G1978B Multimode using Multiple FIA
method in Mixed Mode operation
Preparation
1 Do an autotune if necessary.
After the autotune has completed, you may need to wait up to 30 minutes
before continuing to allow the calibrant solution to be pumped out of the
Quad instrument. This minimizes any background signal from the calibrant.
You may need to further optimize the nebulizer pressure to achieve
maximum instrument sensitivity.
2 Load the method MMCheckSL_ES.M and:
a Edit the method to ensure that 65:35 methanol/water with .2% acetic acid
is selected as the LC solvent.
b Save the method.
3 Repeat for MMCheckSL_CI.M and MMCheckSL_MX.M.
4 Place the vial into the LC autosampler.
• Position 21: vial with the ESI + APCI LC Demo Sample (p/n
G1978-85000)
Create and run a Multiple FIA Method Sequence
1 Select RunControl > Run Multiple FIA Methods.
2 In the Run Multiple FIA Methods dialog box, click Group > Add Group.
3 Type in a unique directory name for the data files to be stored.
4 In the Run Multiple FIA Methods dialog box, click Methods > Add Method,
select MMCheckSL_ES.M and click OK.
5 Repeat to add MMCheckSL_CI.M.
6 Repeat to add MMCheckSL_MX.M.
7 Click Data File for the MMCheckSL_ES.M data file, then:
a Change the Subdirectory value to a unique subdirectory name in which
to store the data files.
b Change Data File to Multi_ES.
c Change Operator to the user’s name or identification code.