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Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 23
Verification 2
To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes
Sensitivity Verification for Multimode in ESI Positive SIM Mode
1 Start up the ChemStation software.
2 Change the view to MSD Tune, and start an Autotune.
After the autotune completes, you may need to wait up to 30 minutes to
allow for the calibrant solution to be pumped out of the Quad instrument.
This minimizes any background signal resulting from the calibrant.
3 Change the view to Method and Run Control, and load the method
6120SIMES_MM.M (6120 model).
4 Edit the method to ensure that 75:25 methanol/water with 0.1% formic or
acetic acid is selected as the LC solvent.
5 Place the vials into the LC autosampler.
• Position #1: empty, uncapped vial
• Position #2: vial of the solvent used for dilution (solvent blank)
• Position #3: vial with the reserpine sample (2 pg/µL)
6 Run the method.
The method does an FIA run with one injection of the empty vial, five
injections of the solvent blank, and five injections of the reserpine sample.
You may need to further optimize the nebulizer pressure by running FIA
experiments to achieve maximum instrument sensitivity.
7 Review the results.
Method name 6110SIMES_MM.M
(6110 Quad)
6120SIMES_MM.M
(6120 Quad)
6110SIMCI_MM.M
(6110 Quad)
6120SIMCI_MM.M
(6120 Quad)
Performance Specification 20: 1 pk-pk
100: 1 rms
10: 1 pk-pk
50: 1 rms
Table 1 6110 and 6120 Quad Performance Verification Summary Table
G1978B
ES Positive SIM Mode
G1978B
APCI Positive SIM Mode