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Table of Contents
6.4 Single Wavelength (absorbance, concentration and transmittance measurements) ....................................... 87
6.4.1 Set up single wavelength mode .............................................................................................................. 87
6.4.2 Take single wavelength measurements (single reading) ........................................................................ 89
6.4.3 Take single wavelength measurements (continuous readings) .............................................................. 89
6.5 Multi-Wavelength mode – measurements at more than one wavelength ........................................................ 90
6.5.1 Set the reading mode at different wavelengths ....................................................................................... 90
6.5.2 Complete a measurement in the multi wavelength mode ....................................................................... 93
6.6 Wavelength Scan mode – recording of absorbance and transmittance spectrums ......................................... 94
6.6.1 Set up the wavelength scan .................................................................................................................... 94
6.6.2 Wavelength scan reading ....................................................................................................................... 97
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis .................................. 98
6.6.3 Work with reference scans ...................................................................................................................... 99
6.7 Time course of absorbance/transmittance ..................................................................................................... 101
6.7.1 Time course setup parameters ............................................................................................................. 101
6.7.2 Time course scan reading ..................................................................................................................... 102
6.7.3 Analysis of time course data ................................................................................................................. 103
6.7.3.1 Navigation of a time scan or a time scan analysis .................................................................... 104
6.8 System checks ............................................................................................................................................... 105
6.8.1 Instrument information .......................................................................................................................... 105
6.8.2 Upgrade of the instrument software ...................................................................................................... 106
6.8.3 Optical checks ....................................................................................................................................... 106
6.8.3.1 Wavelength check .................................................................................................................... 107
6.8.3.2 Noise check .............................................................................................................................. 108
6.8.3.3 Stray light check ....................................................................................................................... 110
6.8.3.4 Absorbance check .................................................................................................................... 111
6.8.3.5 Drift check ................................................................................................................................. 112
6.8.3.6 Verification kit ........................................................................................................................... 113
6.8.4 Output checks ....................................................................................................................................... 115
6.8.5 Lamp history ......................................................................................................................................... 115
6.8.6 Factory service ...................................................................................................................................... 116
6.8.7 Service time .......................................................................................................................................... 116
6.8.8 Instrument Backup ................................................................................................................................ 117
Section 7 Maintenance ...................................................................................................................................... 121
7.1 Cleaning requirements ................................................................................................................................... 121
7.1.1 Spectrophotometer ............................................................................................................................... 121
7.1.2 Display .................................................................................................................................................. 121
7.1.3 Cuvettes/sample cells ........................................................................................................................... 121
7.2 Lamp replacement ......................................................................................................................................... 122
7.2.1 Tungsten lamp replacement ................................................................................................................. 122
7.2.2 Deuterium lamp (UV) replacement ....................................................................................................... 123
7.3 Fuse replacement .......................................................................................................................................... 124
7.4 Filter pad maintenance .................................................................................................................................. 125
7.4.1 Filter pad replacement .......................................................................................................................... 125
Section 8 Troubleshooting ................................................................................................................................ 127
Section 9 Replacement Parts ............................................................................................................................ 129
Section 10 Contact Information ........................................................................................................................ 131
Section 11 Limited Warranty ............................................................................................................................. 133
Index .................................................................................................................................................................... 135