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BioOptix™ 10
Section 2 Installation
2-12
Buffer B – To form gradient buffers during purification, connect
Buffer B solution(s) to these ten inlet ports. Perform the steps
described in Buffer A above to complete these fluid connections.
Sample In – The ferrules on these inlet ports accept the
0.063" OD tubing. The accessory kit contains Teflon tubing of
this size in five different colors. Cut the tubing to length
(standard length is 36" or 91 cm), insert one end into a Sample In
port, and route the other end to the sample container.
Note
Although not critical for operation, installing the colored tubing
according the chart below may be helpful should there be a
later need to trace the lines or monitor system performance.
Waste Out – The ferrules on these inlet ports accept the
0.063" OD tubing listed in Table 2-1, found in the accessories kit.
Cut the tubing to length (standard length is 36" or 91 cm). Then,
while observing the color-coded channel numbers, insert one end
into a Waste Out port, and route to a suitable collection point.
If there might be a need to recover waste fluid (e.g. undetected
purified sample), a collection container for each channel is recom-
mended. Isolating waste fluid for each channel can simplify
sample recovery.
Pump Purge – This port is used when the pumps clears any
fluid remaining in the pump. This fluid is cleared before a run is
started or during system maintenance. Connect a length of
0.250" OD Teflon tubing (P/N 023-0508-01) and use it to route
the pump purge fluid to a suitable collection container. The
standard length of tubing is 60" or 152 cm.
Column Positions – For initial operation, and when channels
are unused during operation, tubing must be inserted between
the Column Inlet and Outlet ports. Use the column bypass tube
(P/N 60-5203-170) or pieces of clear 0.063" ID tubing (P/N
023-0505-09) from the bulk roll in accessory kit.
Foxy – The two bundles of tubing on the right side of the
BioOptix 10 connect to the Foxy 200 Fraction Collector. Each
color-coded tube is labeled with its channel number and finished
with a threaded fitting.
Table 2-1 Tubing Color chart
Channel Numbers Color Part Number
1, 6 Blue 023-0505-06
2, 7 Yellow 023-0505-04
3, 8 Orange 023-0505-03
4, 9 Green 023-0505-05
5, 10 Clear 023-0505-09