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BioOptix™ 10
Section 3 Operation
3-12
3. Select a wash fluid. The wash fluid must be compatible
with the buffer system and of sufficient strength to dis-
solve salts. If this solution is different than the buffers,
move the Buffer In lines to the wash fluid container.
4. For all selected channel positions, turn the Mode knob to
the purge pump position.
5. Click the Start button to begin the flush routine.
The BioOptix 10 flushes the pumps.
6. When the routine is finished, return the Mode knobs to the
Operate position.
The flush routine can be modified to meet your application’s
needs. To modify the pump flush select the Tools>Edit Pump Flush
menu command. This command opens a window that allows you
to change the flush volume, flow rate, Buffer A and B mixing, and
the stroke volume. Click the Save button to save your changes.
3.8.2 Saving Run Data The BioOptix 10 software automatically saves the run data and
chromatograms using the experiment name as the filename. Two
types of files are saved: “.lst” and “.run” files.
This run data can be retrieved and viewed at a later time using
BioOptix 10 software. To do so, select the File>Open menu
command or click the Open toolbar button. Multiple channels
from the experiment can be opened by select the “.lst” file. Indi-
vidual channels can be viewed by opening a “.run” file.
Viewing the run data and chromatograms is very similar to the
operation displays described in section 3.7.2.
The run data and chromatograms displayed in the active window
may be exported in various formats for archiving and sharing.
• Hard copies can be produced by selecting the File>Print
menu command or clicking the Print toolbar button.
Before printing you can also change the page setup
(File>Page Setup…) and preview the page (File>Print
Preview).
• Other file formats can be produced using the File>Save
As menu command. These formats are:
❍ DOC–Microsoft Word format that includes the run
data and chromatogram.
❍ RTF–Rich Text Format that includes the run data
and chromatogram. This format can be opened with
many word-processing programs.
❍ PDF–Portable Document Format that is an
electronic file similar in appearance to the hard copy
output. Viewing PDF files require Adobe’s Acrobat
Reader, a free download from www.adobe.com.
❍ TXT–Text-only format that lists the run parameters
and history. This format does not support
chromatogram data.