B-1
BioOptix™ 10
Appendix B Fluid Path Diagrams
This appendix contains simplified diagrams of the plumbing and
valves internal to the BioOptix 10 system. Various operating
states are depicted for a single channel. A complete plumbing
diagram can be found in Figure B-7.
An understanding of fluids paths can be helpful when designing
custom template files (Appendix A), manually controlling the
system, or troubleshooting.
The valves in the diagrams are:
• Buffer–This valve switches between Buffer A and
Buffer B to produce the correct mix (%B) of buffer
solution.
• Sample–This valve allows the pump to draw either
sample from the Sample In ports or buffer solution.
• Channel–This valve controls allows fluid to be pumped
only when the channel is active.
• Mode–This manually-controlled front-panel valve has
three positions:
❍ Operate–passes fluid from the pump to the column.
❍ Manual Inject–directs injected fluid onto the column.
❍ Purge Pump–diverts fluid from the pump to a purge
port that is common to all channels.
• Effluent–This valve sends the effluent to the Foxy
fraction collector or to the channel’s waste port.
B.1 Fluid-type Blocks Fluid blocks pump buffer solution through the column (Figure
B-1).
Figure B-1 Fluid path for Fluid-type blocks
Buffer
Sample
Channel
Mode
Effluent
Pump
To Waste
To Foxy
Sample In
Buffer A Buffer B
To Pump Purge
Manual Sample Injection
Column