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BioOptix™ 10
Section 3 Operation
3-7
• Collect Effluent – Select the desired action for effluent
fluid as it comes off the column. If Ye s is selected, enter
the desired fraction size.
Note
The Elute tab is a “Gradient” block type. See Appendix A.
3.5.5 Flush Settings This block pumps fluid to flush remaining sample from the
column and system tubing. This step can prepare the column for
the next purification run, storage, or disposal.
The Flush tab is a fluid block type, therefore the settings dis-
played on this tab are the same as the Equilibrate and Wash
tabs. Refer to section 3.5.1 for a discussion on the settings for this
tab.
Figure 3-6 Flush tab settings
3.5.6 Saving the Method File To be used in an experiment file, the method file must be saved.
Save the method by selecting the File>Save menu command. Use
a descriptive file name so that it can be located later.
3.6 Experiments An experiment file can be created by:
• selecting the File>New>Experiment menu command,
• pressing the Ctrl+E keyboard buttons,
•or by clicking the E toolbar button.
Before building and displaying the experiment file, you must
select a method file that will be used for all ten channels. In the
Select a Method for Experiment dialog box, locate and highlight
the method file, then click the Open button.
An experiment file displays operating parameters in a table.
Many of the columns in the table are determined by the template
file used to create the method file. (Refer to Appendix A for more
details on the template file.) Default values will be assigned to
the table cells from the method file you’ve chosen.