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BioOptix™ 10
Section 3 Operation
3-10
Figure 3-9 Pause at end of block
If the block is set to collect effluent (Figure 3-4), the fraction col-
lector will deposit the fluid in the collection tubes. If a peak is
detected, the fraction collector automatically advances to the
next row to isolate the fluid. The fraction collector will also auto-
matically advance to the next row of tubes whenever the spec-
ified fraction size has been collected.
If the block is not set to collect effluent (Figure 3-2), the
BioOptix 10 diverts all pumped fluids to the waste ports.
3.7.2 Operation Displays The window displayed during the run has several features that
let you monitor the operation.
At the start of a run, the plot area displays the gradient as a blue
line (right Y-axis). The X-axis displays the volume pumped.
Below the X-axis is a progress bar. As the run progresses, the
absorbance traces is plotted as a red line (left Y-axis).
Zoom and pan To the right of the plot area are several controls that can modify
the display. The Pan/Zoom switch specifies which action to take
when you use the cursor to click and drag across the plot area. In
Zoom mode, clicking and dragging across part of the plot area
will zoom in on the selected region. In Pan mode, clicking and
dragging moves the plot area under the cursor to a new position.
The view can be returned to the default view by clicking the
Reset Graph button.
Absorbance trace In addition to the zoom feature which scales the plot area both
horizontally and vertically, the scale of the absorbance trace can
be modified alone. To change the left Y-axi s S cal e, click and select
a new value from the pull-down list.
Channel positions Channel positions can be displayed in the plot area individually
or collectively. Highlight the channels you wish to display. If mul-
tiple absorbance traces and gradients are selected, they will be
overlaid in the plot area. This can be useful when comparing
channels, especially when desiring to monitor the effects of any
experimental interventions.
Status bar The status bar is located at the bottom edge of the BioOptix 10
window. The fields in this bar display system information and
messages during the run.
3.7.3 Suspending System
Operation
The system automatically stops after completing all blocks.
However, the experiment can be paused at any time by clicking
the Stop button. This stops the pumps which allows you to refill
or change buffers, or make other mechanical adjustments to the
system. Click the Play button to resume the experiment.