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Chapter 3 Peak Detection and Labeling
3-50 Applied Biosystems
3
Example Figure 3-19 and Figure 3-20 illustrate the effects of
deisotoping.
Before deisotoping (Figure 3-19), the spectrum includes an
isotope pattern with four detected peaks.
Figure 3-19 Spectrum Before Deisotoping
After deisotoping (Figure 3-20), the trace includes two
labeled centroid bars that represent monoisotopic masses,
indicating that the original trace represents two isotopic
envelopes. The intensity of a centroid bar is proportional to
the area of all peaks in the isotope cluster.
Figure 3-20 Spectrum After Deisotoping