Chapter 5 Examining Spectrum Data
5-54 Applied Biosystems
5
General
guidelines for
setting
parameters
Refer to the following table to determine how to set Advance
Baseline Correction parameters and obtain the desired
baseline correction.
Condition Set Parameters To
Baseline follows a rising peak cluster • Peak Width—Average peak width
• Flexibility—0.5
• Degree—0.5
Baseline is gently curving • Peak Width—10 to 20 times peak width
• Flexibility—0
• Degree—0.5 (decrease further to raise
the baseline)
Baseline rise should be ignored and
treated as signal
• Peak Width—Half of the width of the
cluster to correct (in the illustration
shown, if the width of the cluster is 200,
set Peak Width to 100)
• Flexibility—0
• Degree—0.5 (decrease further to raise
the baseline)
Peak cluster