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Chapter 6 Using Tools and Applications
6-20 Applied Biosystems
6
6.3 Using the
Mass Resolution Calculator
Calculating mass
resolution
To calculate mass resolution:
1. Display the spectrum of interest. Make sure the
Spectrum window is active.
2. If you are examining Voyager data, baseline correct the
spectrum. The Peak Height used by the Resolution
calculator is calculated from 0. For information, see
Section 5.8.2, Using Baseline Correction.
NOTE: Baselines in Mariner data are typically at 0.
Baseline correction is not necessary.
3. From the Tools menu, select Resolution Calculator.
NOTE: If the Spectrum window is not active, Resolution
Calculator does not appear on the Tools menu.
4. In the Resolution Calculator dialog box (Figure 6-12), set
the percentage of peak height at which to calculate
resolution. The default is 50%, which calculates the
resolution at the full width/half maximum (FWHM) of a
peak.
NOTE: Measuring resolution at the full width/half
maximum is the industry standard. Use 50% Peak
Height for most applications. If a peak is not resolved at
the Peak Height % you set, an error message is
displayed in the Result tab.