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Chapter 6 Using Tools and Applications
6-18 Applied Biosystems
6
Evaluating traces The theoretical isotope distribution is displayed in the
Spectrum window with an ISO trace label (Figure 6-10).
Figure 6-10 Isotope Trace
If you have the Replace mode set to Add in the Display Trace
dialog box, a new trace is added. If you have the Replace
mode set to Replace, the isotope trace replaces the active
trace. For more information on Replace mode, see “Setting
the Replace mode” on page 2-17.
To evaluate the trace, you can:
• Overlay theoretical and observed isotope patterns. See
Section 2.4.8, Overlaying Traces, for information.
• Add new traces and calculate the isotope multiple times
using different settings, then compare or overlay the
different calculations.
Returning to the
original spectrum
To return to the original trace, see “Returning to the original
spectrum” on page 5-3.