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Chapter 9 Troubleshooting
9-10 Applied Biosystems
9
9.4 Calibration Troubleshooting
Table 9-6 Calibration Troubleshooting—Mariner and Voyager
Symptom Possible Cause Action
Auto Calibration is turned
on, but current spectrum
is not auto calibrated
Current spectrum is not
calibrated until the next
time the spectrum is
displayed
Advance to the next
spectrum, then return to
the current spectrum. For
more information, see
Section 5.4, Automatic
Calibration.
During calibration, the
software is not matching
spectrum masses to some
reference masses in the
calibration reference file
The calibration routine
checks peak width to
determine if a peak
matches a Resolved
Isotope Mass or an
Average mass. If narrow
peaks are specified as
Average Masses in the
calibration reference file,
the software mistakes
these narrow peaks as
isotopically resolved and
ignores the reference
mass.
When specifying highly
charged, non-isotopically
resolved species with
peaks less than 1 Da wide
(for example, myoglobin
+20) as reference masses
in a calibration reference
file, set the peak type as a
Resolved Isotope Mass
(even though it is not a
resolved isotope). See
Section 5.3.3, Creating or
Modifying a
Calibration Reference File
(.REF).
Mass tolerance or
Minimum Intensity set too
high
Adjust. See Section 5.3,
Manual Calibration.