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Using the Ladder Sequencing Toolbox
Data Explorer
™
Software User’s Guide C-7
C
7. Under Annotate Spectrum, select the types of labels you
want displayed:
• Reference Mass (*)Mass of the reference
peak against which the current peak is
compared.
• Mass DifferenceDifference between the
current peak and the reference, preceded by a
minus sign (–).
• Peak MassMass of the current peak,
preceded by an equal sign (=).
8. Click Label Peaks.
If Peptide is selected, the software:
• Examines the spectrum in 55 Da increments
and selects the most intense ion in the range.
(The 55 Da increment is used because it is
less than the smallest mass difference related
to a residue.)
• Labels mass differences (plus or minus the
specified Tolerance) that correspond to amino
acids.
• Labels the reference peak from which the
mass difference was derived with an
asterisk (*).
• Applies the additional labels you selected
under Annotate Spectrum.