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Chapter 5 Examining Spectrum Data
5-52 Applied Biosystems
5
Flexibility With the Peak Width parameter, determines the number
of points used to estimate the baseline amplitude at
regularly spaced points in the spectrum, but is not directly
proportional to the number of points used. Valid entries
are 0 to 1.0.
The default value of 0.5 works best for most applications.
A value closer to 0 reduces flexibility and provides a
smoother more generalized baseline correction (see
below).
A value closer to 1 provides more localized baseline
correction by using a larger number of points to estimate
the baseline (see below).
If the baseline rises adjacent to peaks after
baseline-correcting, decrease the Flexibility value and
baseline-correct again.
(continued)
Parameter Description/Specifies
Flexibility=1Flexibility=0