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Chapter 7 Data Explorer Examples
7-4 Applied Biosystems
7
7.1.2 Deconvoluting and
Evaluating Unresolved
Chromatographic Peaks
Overview You can use the Data Explorer software to deconvolute
chromatographic peaks and obtain masses for each
component by:
• Creating a combined spectrum to identify the coeluting
species by mass
• Creating extracted ion chromatograms for the masses,
and comparing the extracted ion chromatograms to the
original TIC containing the unresolved peaks
You can then create a combined spectrum from each
extracted ion chromatogram to evaluate the spectral data.
Creating a
combined
spectrum
The following example illustrates how to deconvolute two
unresolved chromatographic peaks in a tryptic digest of
cytochrome c:
1. Display the data file.
2. In the Chromatogram window, zoom in on the unresolved
peaks (Figure 7-2).
Figure 7-2 Unresolved Peaks in Cytochrome C
3. In the Chromatogram window, right-click-drag over the
unresolved peak pair.