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Table of Contents
vi Applied Biosystems
Chapter 4 Examining Chromatogram Data
4.1 Overview .............................................................................. 4-2
4.2 Creating an Extracted Ion Chromatogram
..................................... 4-5
4.2.1 Creating an Extracted Ion Chromatogram (XIC) ..................... 4-5
4.2.2 Creating a Constant Neutral Loss (CNL) Chromatogram ........ 4-9
4.3 Creating an Extracted Absorbance Chromatogram (XAC)
(Mariner Data Only)
................................................................ 4-13
4.4 Noise Filtering/Smoothing
........................................................ 4-17
4.5 Adding and Subtracting Raw Spectra Within a Data File
.................. 4-20
4.6 Displaying MS Method Data (Mariner Data Only)
........................... 4-23
4.7 Adjusting the Baseline
............................................................ 4-27
4.7.1 Using Baseline Offset ...........................................................4-27
4.7.2 Using Baseline Correction .....................................................4-29
4.8 Using UV Trace Offset (Mariner Data Only)
.................................. 4-30
Chapter 5 Examining Spectrum Data
5.1 Overview .............................................................................. 5-2
5.2 Creating a Combined Spectrum
................................................. 5-4
5.3 Manual Calibration
.................................................................. 5-5
5.3.1 Overview of Manual Calibration ............................................. 5-5
5.3.2 Manually Calibrating .............................................................. 5-7
5.3.3 Creating or Modifying a Calibration Reference File (.REF) ....5-17
5.3.4 Reverting to Instrument Calibration .......................................5-22
5.3.5 Hints for Calibrating Mariner Data .........................................5-24
5.3.6 Hints for Calibrating Voyager Data ........................................5-25
5.4 Automatic Calibration
.............................................................. 5-26
5.4.1 Overview of Automatic Calibration ........................................5-26
5.4.2 Importing and Specifying Automatic Calibration Settings .......5-29
5.4.3 Automatically Calibrating (Mariner Data Only) .......................5-34