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Chapter 5 Examining Spectrum Data
5-50 Applied Biosystems
5
3. Enter parameters as described below. These parameters
interact with each other and require experimentation to
determine the optimum settings for your data. Refer to
“General guidelines for setting parameters” on page 5-54
for more information.
Parameter Description/Specifies
Peak Width
(at half height)
Value the software uses to estimate the baseline
amplitude at regularly spaced points in the spectrum. The
number of regularly spaced points used is derived using
the Peak Width parameter and the Flexibility parameter.
The illustration below shows how using a higher number
of points estimate the baseline (accomplished by using
higher Peak Width and Flexibility settings) can affect the
shape and slope of the calculated baseline.
(continued)
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Lower number of points
Higher number of points