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Chapter 5 Examining Spectrum Data
5-18 Applied Biosystems
5
Definition A calibration reference file (.REF) is a list of masses and
corresponding information from which you can select
reference masses during calibration.
.REF files
provided
The following default calibration reference files are provided
with the software:
• MARINER_POS.REF
• MARINER_NEG.REF
• VOYAGER.REF
• ANGIOTENSIN_FRAGMENTS.REF
• IMMONIUM_IONS.REF
Calibration
reference
file contents
A calibration reference file is a tab-delimited text file that
contains the following columns of information:
• Reference mass
• Charge (defaults to 0 if you do not specify)
• Type—Resolved, Average, or Unknown (defaults to
Resolved if you do not specify)
• Name (optional)
• Elemental composition (optional)
NOTE: If you view the reference file in a text editor (such as
Notepad Editor), the order of the information listed in the
reference file is different than the order of the information
displayed in the Reference Mass dialog box (see Figure 5-3
on page 5-10).
Creating and
saving a
calibration
reference file
NOTE: You can also create a reference file using the Ion
Fragmentation calculator. For more information, see
“Creating a calibration reference file (.REF)” on page 6-30.
To create and save a calibration reference file:
1. From the Process menu, select Mass Calibration, then
select Edit/Create Reference File.