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Peak Labeling
Data Explorer
™
Software User’s Guide 3-53
3
3.5.1 Charge State Labels
Charge state
labels
A unique feature of the Data Explorer software is the ability to
label multiply charged isotope peaks with their charge state.
In general, the observed spacing between isotopes is
determined by the charge state of the detected ion. Spacing
between isotope peaks is narrower at higher charge states.
For more information on isotopes, see Appendix B, Overview
of Isotopes.
To determine charge state, the software uses the following
charge state determination parameters:
• Max Charge State
• Max Isotope #
• Min and Max Intensity
For a description of these parameters, see “Advanced
Settings (spectrum data only)” on page 3-28 and “Charge
State Determination and Examples” on page 3-32.
NOTE: If Charge State Determination parameters are set
incorrectly, the charge state is determined incorrectly. For
more information, see “Charge state parameter examples”
on page 3-32 and “Charge State and Isotope Determination
Troubleshooting—Mariner Only” on page 9-18.
Requirements To label isotope peaks with charge states, the following
must be true:
• Mass must be within the range in which the system can
resolve the isotope peaks:
• <4,000 Da on a Mariner system
• <5,000 Da on a Voyager-DE PRO system
• <6,000 Da on a Voyager-DE STR system