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Appendix B Overview of Isotopes
B-4 Applied Biosystems
B
Figure B-3 Mass Spectrum of Angiotensin I at
Resolution 3,000
In compounds with more than 100 carbon atoms, the height of
the first
13
C isotope peak exceeds the height of the
12
C peak.
Isotope-limited
resolution
The mass range you analyze and the resolving power of the
analyzer determine if you observe resolved isotope peaks in a
mass spectrum.
Figure B-3 represents angiotensin I at a resolution of 3,000.
Isotopes are fully resolved, and sharp peaks are observed in
the mass spectrum.
At lower resolutions, the shape of the individual peaks
becomes less pronounced, or may not be differentiated at all.
Figure B-4 represents angiotensin I at a theoretical resolution
of 1,000.
M
+
(M+1)
+
(M+2)
+