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Chapter 5 Examining Spectrum Data
5-32 Applied Biosystems
5
6. Specify reference masses to add by doing either of the
following:
• Click a mass, then click OK.
• Type new reference mass information in the
Name, Theoretical m/z, Charge, and Elemental
Composition fields, then click OK.
NOTE: You must type a minus sign (–) preceding
the charge in the Charge text box for negative
charge states.
NOTE: You can save changes to the Calibration
Reference File by clicking Save. For more
information, see “Modifying a calibration reference
file” on page 5-20.
The Automatic Calibration Settings dialog box is
displayed again (see Figure 5-7 on page 5-30).
7. Repeat step 5 and step 6 to add all needed masses.
8. To delete masses, do either of the following:
• Select a reference, then click Delete Selected
Reference
• Click Delete All References
9. Enter Reference Matching Criteria:
• Minimum Intensity—Peaks must be above this
intensity to be considered a match. Select the unit
for Minimum Intensity, % Relative Intensity or
Absolute Counts.
• Mass Tolerance—Peaks must be within this
tolerance of the theoretical m/z to be considered a
match. Select the unit, m/z or ppm.