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Chapter 4 Examining Chromatogram Data
4-20 Applied Biosystems
4
4.5 Adding and Subtracting
Raw Spectra Within a Data File
Use the Add/Subtract Spectra command to manipulate raw
spectra within a single data file.
NOTE: To manipulate processed spectra, spectra from
different data files, or spectra acquired under slightly
different instrument calibrations, use Trace Arithmetic. For
more information, see Section 5.12, Adding and Subtracting
Raw or Processed Spectra from the Same or Different Data
Files (Dual Spectral Trace Arithmetic).
Adding and
subtracting
spectra
To add and subtract spectra:
1. Click the Spectrum window to activate it.
2. Select Duplicate Active Trace from the Display menu to
keep the original data displayed after processing.
3. From the Process menu, select Add/Subtract Spectra.
The Add and Subtract Spectra dialog box is displayed
(Figure 4-9).