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Mass Deconvolution (Mariner Data Only)
Data Explorer
™
Software User’s Guide 5-37
5
5.6 Mass Deconvolution
(Mariner Data Only)
NOTE: Mass deconvolution is not supported for
Mariner DAD data.
NOTE: The Mass Deconvolution software is an option in the
Data Explorer software. The Multiple Charge command on
the Process menu is dimmed if you have not purchased the
option.
Overview You can use the Data Explorer Mass Deconvolution features
to generate a theoretical zero-charge spectrum that
represents the molecular mass of a protein:
• Mass Deconvolution—Use when you have a
spectrum with clearly resolved, multiply charged peaks.
You specify the m/z values for the peaks to include.
This function requires at least two adjacent peaks within
the same charge envelope.
• Convert to Zero-Charge Spectrum—Use when you
have a spectrum with overlapping charge envelopes, or
a noisy baseline. You specify a mass range for the
peaks to include and a mass range for the molecular
mass.
Mass
Deconvolution
To use the Mass Deconvolution function:
1. Click the Spectrum window.
2. Select Duplicate Active Trace from the Display menu to
keep the original data displayed after processing.
3. From the Process menu, select Multiple Charge, then
select Mass Deconvolution.