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Chapter 9 Troubleshooting
9-20 Applied Biosystems
9
Spectrum peaks not
labeled with charge state
when charge state labels
are selected
Mass of original molecule
above 4,000 Da, not
range in which the Mariner
system can resolve the
isotope peaks
No action. Normal
occurrence.
Charge State parameters
not set to detect at least
two isotope peaks
Adjust parameters. See
“Peak Processing
parameters (spectrum
data only)” on page 3-26.
Peaks are more than 1 Da
apart
No action. Normal
occurrence.
Peaks are not from the
same isotope species
No action. Normal
occurrence.
Filter width is set too high
to detect other isotope
peaks
Adjust Filter Width. See
“Advanced Settings
(spectrum data only)” on
page 3-28.
Maximum Charge State
for charge state
determination is set lower
than the charge state of
the peak
Adjust the Maximum
Charge State. See “Peak
Processing parameters
(spectrum data only)” on
page 3-26.
Table 9-12 Charge State and Isotope Determination Troubleshooting—Mariner
Only (Continued)
Symptom Possible Cause Action