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Chapter 7 Data Explorer Examples
7-18 Applied Biosystems
7
7.2.3 Detecting Peaks
from Complex Digests
Overview Complex digests often contain hundreds of peaks which may
have relatively low signal-to-noise ratios. To quickly screen out
noise and detect peaks of interest:
• Noise filter/smooth to remove initial noise.
• Set initial peak detection thresholds low enough to detect
all peaks, to ensure that monoisotopic peaks are detected
for proper deisotoping.
• Deisotope to identify isotope peak clusters. The
deisotope function amplifies peak intensity based on the
total area of all peak areas in the isotope cluster. It does
not amplify noise peaks.
• Increase peak detection thresholds to eliminate noise
peaks.
Figure 7-18 represents a spectrum trace from a digest.
Overlapping Peak Labels are enabled to illustrate the large
number of peaks detected in the spectrum (over 300 peaks
contained in the peak list displayed in the Output window).
Figure 7-18 Digest with Several Hundred Peaks
Detected (Allow Overlapping Labels Enabled)