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Chapter 4 Examining Chromatogram Data
4-12 Applied Biosystems
4
Example Figure 4-4 shows a TIC that contains three flavonoid
compound peaks. To determine if the diglycosyl group has
fragmented from the parent ion in any of these compounds,
you can generate a CNL extracted chromatogram.
Figure 4-4 TIC for Flavonoid Mixture Containing Three Peaks
Figure 4-5 shows a CNL extracted chromatogram generated
for mass difference of 308.146 m/z which corresponds to the
diglycosyl group.
Figure 4-5 CNL Containing Two Peaks
The CNL extracted chromatogram contains only two peaks,
indicating that the diglycosyl group has fragmented from the
parent ion in the first two compounds only.