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Chapter 5 Examining Spectrum Data
5-34 Applied Biosystems
5
5.4.3 Automatically Calibrating
(Mariner Data Only)
This section includes:
• Automatically calibrating
• Applying new constants to the data file
• Calibration results
• Applying auto calibration settings to other files
Automatically
calibrating
To automatically calibrate:
1. Open the data file to calibrate.
2. Click the Spectrum window.
3. From the Process menu, select Mass Calibration, select
Auto Calibrate State, then select On.
NOTE: The Auto Calibrate State command setting is
stored in a .DAT file. That is, if you turn on this
command, then close the .DAT file, the command is on
the next time you open the .DAT file.
NOTE: The Auto Calibrate State command is dimmed
unless you specified automatic calibration settings in
the data file. See Section 5.4.2, Importing and
Specifying Automatic Calibration Settings.
4. Display the spectrum of interest.
The spectrum is calibrated and displayed with an AC
trace label if calibration is successful. Calibration
results are displayed in the Output window.
If calibration fails, the spectrum is displayed with an
AC-failed trace label.