Table of Contents
viii Applied Biosystems
Chapter 7 Data Explorer Examples
7.1 Mariner Data Examples ............................................................ 7-2
7.1.1 Improving Signal-To-Noise Ratio ............................................ 7-2
7.1.2 Deconvoluting and Evaluating
Unresolved Chromatographic Peaks .......................................7-4
7.1.3 Determining if a Peak is Background Noise ............................ 7-8
7.2 Voyager Data Examples
.......................................................... 7-11
7.2.1 Detecting and Labeling Partially Resolved Peaks ..................7-11
7.2.2 Processing Before Calibrating to Optimize Mass Accuracy ...7-14
7.2.3 Detecting Peaks from Complex Digests ................................7-18
Chapter 8 Viewing Voyager PSD Data
8.1 Displaying PSD Data ............................................................... 8-2
8.2 Applying Fragment Labels
......................................................... 8-8
8.3 Calibrating a PSD Spectrum
..................................................... 8-10
8.3.1 Checking Peak Detection ......................................................8-11
8.3.2 Calibrating ............................................................................8-12
8.3.3 Creating PSD Calibration (.CAL) Files and
Applying to Other Data Files .................................................8-20
8.3.4 Creating PSD Calibration Reference (.REF) Files .................8-21
8.3.5 Changing the Precursor Mass ...............................................8-23
Chapter 9 Troubleshooting
9.1 Overview .............................................................................. 9-2
9.2 General Troubleshooting
.......................................................... 9-3
9.3 Processing, Tools, and Applications Troubleshooting
....................... 9-6
9.4 Calibration Troubleshooting
...................................................... 9-10
9.5 Printing Troubleshooting
.......................................................... 9-14
9.6 Peak Detection and Labeling Troubleshooting
............................... 9-15