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Chapter 5 Examining Spectrum Data
5-24 Applied Biosystems
5
5.3.5 Hints for Calibrating Mariner Data
Ensuring that
masses match
during calibration
Mariner TOF Analyzer parameters affect flight times of ions. If
you acquired Mariner data using different TOF Analyzer
parameters, and did not calibrate the data in the Instrument
Control Panel to compensate for the altered parameters,
masses in the data file may be significantly different from the
reference masses. For information on calibrating in the
Instrument Control Panel, see the Mariner Workstation
User’s Guide.
Before performing automatic matching, set the Mass
Tolerance and Minimum Intensity appropriately to match the
expected reference masses. After automatic matching, check
the list of matched peaks to make sure the correct reference
masses and peak masses are selected.