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Converting to a Singly Charged Spectrum (Mariner Data Only)
Data Explorer
™
Software User’s Guide 5-59
5
5.10 Converting to a Singly Charged
Spectrum (Mariner Data Only)
NOTE: Single-charge conversion is not supported for
Mariner DAD data.
Description The Single-Charge Conversion function generates a
theoretical, centroided, singly charged spectrum. This function
uses isotopic spacing in detected spectral peaks to generate
the theoretical spectrum.
For example, if you use this function on a spectrum that
includes +3 peaks, the software multiplies each mass by 3 and
removes the mass of two extra protons. The resulting
spectrum contains theoretical +1 species.
If more than one charge state is present, each charge state is
converted appropriately, and the total range of the converted
spectrum is approximately 1.2 times the m/z of the highest m/z
present.
Requirements Use this function only on isotopically resolved data that is
labeled with the correct charge state. Peaks with low
signal-to-noise ratios may be labeled with incorrect charge
states. Set peak detection thresholds to disregard these peaks
before converting the spectrum.
If you use this function on a spectrum that is not correctly
labeled, the resulting spectrum may contain charge states
other than 1.
Converting To convert a multiply charged spectrum to a singly charged
spectrum:
1. Display the spectrum trace of interest.
2. Examine the spectrum. Make sure peaks are labeled with
the correct charge state, and that no noise peaks are
labeled with a charge state. Adjust peak detection
parameters as needed.