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Chapter 5 Examining Spectrum Data
5-64 Applied Biosystems
5
5.12 Adding and Subtracting Raw or
Processed Spectra from the
Same or Different Data Files
(Dual Spectral Trace Arithmetic)
The Dual Spectral Trace Arithmetic function lets you add two
spectra together, or subtract one spectrum from another.
Spectra can be raw or processed, and can be from the same
or different data files.
To use the Dual Spectral Trace Arithmetic function:
1. To perform this function on spectra from different data
files, copy a spectrum trace from one data file to
another. See Section 2.5.2, Copying Traces from
Multiple Data Files to a Window, for information on
copying traces.
2. Activate the Spectrum window that contains the two
traces of interest.
3. Process the traces as needed.
4. Click the first trace in the Spectrum window to make it the
active trace.
NOTE: The Dual Spectral Trace Arithmetic command is
dimmed unless the first trace in the Spectrum window is
the active trace.
5. From the Process menu, select Dual Spectral Trace
Arithmetic.
The Dual Spectral Trace Arithmetic dialog box is
displayed (Figure 5-23).